MGMCRI, SBV

Mahatma Gandhi Medical College and Research Institute (MGMCRI)

A constituent college of

Sri Balaji Vidyapeeth (SBV), PUDUCHERRY

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Clinical Diagnostic laboratory services

The microbiology laboratory caters to the needs of the various clinical specialities providing a wide range of diagnostic microbiological investigations for diagnosis of several infectious and immunological conditions. The laboratory has applied for NABL accreditation.

Investigations Performed in Diagnostic Labs

Bacteriology

  • Gram’s staining, Albert’s staining, modified acid-fast staining.
  • Culture and sensitivity- carried out inside the Laminar flow bench.
  • Combination disc method for ESBL detection.
  • Modified Hodge test and EDTA disc synergy test for carbapenemases and MBL detection.
  • Slide agglutination test using specific anti-sera for identification of important pathogens.
  • Anaerobic culture.

Immunology

  • Rheumatoid factor latex agglutination.
  • CRP latex agglutination.

Serology

  • Widal Test for enteric fever.
  • ASO (Latex Agglutination) for Streptococcal infection.
  • RPR for Syphilis.
  • Immunochromatographic test for detection of Leptospira IgG & IgM antibodies.
  • Scrub Typhus – ICT & ELISA.

Virology

  • Viral load by RT-PCR (Bio-Rad) for HBV, HCV, CMV.
  • Immunochromatographic test for detection of Dengue IgG & IgM antibodies and NS1 antigen.
  • Rapid test for HCV antibody detection.
  • HBsAg ELISA and rapid test.
  • 4th generation ELISA and Dot ELISA for HIV I & 2.

Tuberculosis

  • Ziehl-Neelsen Method of Staining Acid-Fast Bacilli.
  • Modified Ziehl-Neelsen Staining for lepra bacilli.
  • Auraminerhodamine staining for Mycobacterium tuberculosis.
  • Culture of Mycobacterium tuberculosis.

Parasitology

  • Saline, Iodine and LPCB wet mount for parasitic eggs, cyst and trophozoites.
  • Modified Acid Fast Staining for Cryptosporidium, Isospora and Cyclospora.
  • Leishman’s staining.
  • Immunochromatographic tests for Plasmodium spp.
  • Quantitative Buffy Coat (QBC) examination for malarial parasite identification .
  • IgG ELISA for Neurocysticercosis.

Mycology

  • 10% KOH examination.
  • Modified India ink method.
  • Lactophenol cotton blue (LPCB) mount.
  • Culture on Sabouraud’s dextrose agar with or without cycloheximide.
  • Culture of dermatophytes on Dermatophyte test medium.
  • Slide culture.
  • Germ tube test.
  • Cornmeal agar culture for demonstration of chlamydospores.
  • Antifungal susceptibility testing for yeast.

Molecular Diagnostic Lab

  • PCR for detection of ESBL genes.
  • Staphylococcus aureus mecA gene detection by PCR .
  • Viral load by RT-PCR (Bio-Rad) for HBV, HCV, CMV.
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