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The Department of Microbiology had had a modest beginning in March 2002 rendering diagnostic microbiology services, sharing a common lab along with clinical biochemistry and clinical pathology.

Currently it has developed its diagnostic services as well as improved teaching facilities to cater to an increased strength of 150 MBBS students, apart from BDS, B.Sc. Nursing, Post Basic Nursing, MD Microbiology and M.Sc. Medical Microbiology students. It offers quality diagnostic services to the various clinical departments at an affordable cost.

The microbiology department has three main roles:

  • Teaching/ Academic activities
  • Diagnostic laboratory services
  • Research activities
     

 


The department has a very good infrastructure for both under-graduate and post-graduate teaching and research activities. The department has 4 computers with LAN connection.

Student's lab and Demonstration rooms
The Student's Practical lab is spacious and furnished with 90 student microscopes. The department has two well furnished demonstration/ tutorial rooms. Epidiascope, slide projector and over head projectors are available.

Seminar room cum Departmental Library
The departmental library has 174 medical books catering to the needs of both the undergraduates and the postgraduates. It is very spacious with a large oval table and a seating capacity for 30 people. It has a computer with internet connection and a digital microscope with a 5 megapixel camera.

Museum

The department has an excellent museum with a vast collection of different specimens, charts, models etc (totaling 254) contributing immensely to under-graduate and post-graduate teaching. The medical students are encouraged to visit the museum.

Diagnostic labs

Bacteriology
The diagnostic microbiology lab is fully functional with separate bacteriology, immunology, virology, serology, tuberculosis, mycology and parasitology laboratories.

  • Rapid test for HCY antibody detection
  • HBsAg ELISA and rapid test

The bacteriology lab consists of exudate, urine, blood, stool and anaerobic sections.
Investigations performed

  • Gram's staining, Albert's staining, modified acid fast staining
  • Culture and sensitivity- carried out inside the Laminar flow bench.
  • Combination disc method for ESBL detection
  • Modified Hodge test and EDTA disc synergy test for carbapenemases and MBL detection
  • Slide agglutination test using specific anti-sera for identification of important pathogens
  • Anaerobic culture

Immunology Investigations performed

  • Rheumatoid factor latex agglutination
  • CRP latex agglutination

Serology
Investigations performed:

  • Widal Test for enteric fever
  • ASO (Latex Agglutination) for Streptococcal infection
  • RPR for Syphilis
  • Immunochromatographic test for detection of Leptospira IgG & IgM antibodies

Virology Investigations performed

  • Immunochromatographic test for detection of Dengue IgG & IgM antibodies and NS 1 antigen
  • 4th generation ELISA and Dot ELISA for HIY I & 2

Special equipments:

  • ELISA washer and reader

Tuberculosis
Investigations performed

  • Ziehl-Neelsen Method of Staining Acid-Fast Bacilli
  • Modified Ziehl-Neelsen Staining for lepra bacilli
  • Auramine rhodamine staining for Mycobacterium tuberculosis
  • Culture of Mycobacterium tuberculosis Special equipments:
  • Fluorescent Microscope
  • BSL CABINET Level 2 with virus burn unit

Parasitology
Investigations performed

  • Saline, Iodine and LPCB wet mount for parasitic eggs, cyst and trophozoites
  • Modified Acid Fast Staining for Cryptosporidium, Isospora and Cyclospora
  • Leishman's staining
  • Immunochromatographic tests for Plasmodium spp.
  • Quantitative Buffy Coat (QBC) examination for malarial parasite identification Special equipments:
  • QBC centrifuge and fluorescent microscope
  • Centrifuge

G. Mycology Investigations performed

  • 10% KOH examination
  • Modified India ink method
  • Lactophenol cotton blue (LPCB) mount
  • Culture on Sabouraud's dextrose agar with or without cycloheximide
  • Culture of dermatophytes on Dermatophyte test medium
  • Slide culture
  • Germ tube test
  • Corn meal agar culture for demonstration of chlamydospores Special equipments:
  • BOD Incubator for isolation of fungi
  • BSL Cabinet level 2 with virus burn unit.

Molecular Diagnostic Lab
Housed in the Central Clinical laboratory (Ground floor¬ block of the Hospital)
Investigations performed

  • PCR for detection of ESBL genes
  • Staphylococcus aureus mecA gene detection by PCR Special equipments:
  • Thermocycler
  • Mini Spin Microcentrifuge
  • Gel documentation system with electrophoresis chamber Midi PCR workstation- Deep freezer (-20°C)
  • Laminar flow bench
  • Cooling centrifuge

Research lab
There is a well equipped research lab with all the facilities to support various research activities.

 


Clinical

Diagnostic laboratory services
The microbiology laboratory caters to the needs of the various clinical specialties providing a wide range of diagnostic microbiological investigations for diagnosis of several infectious and immunological conditions. The laboratory has applied for NABL accreditation

Teaching

  • Undergraduate teaching
  • MBBS
  • BDS
  • Nursing
  • Postgraduate teaching
  • MD Microbiology (2009 onwards)
  • M.Sc. Medical Microbiology (2010 onwards)

Awards/ recognition

Dr. Selvaraj Stephen

  • Prof. Stephen S, in view of his expertise, has been nominated by the Director General ICMR as a member of the Expert Group on Rickettsial disease, constituted to steer research in this area.
  • Dr TMA Pai Gold Medal for best research work in Kasturba Medical College, Manipal, 1975.
  • Secured first rank in M.Sc. (Microbiology) Madras University, 1972.

Dr. Joslty M. Easow

  • The Royal College of Pathologists, United Kingdom offered Sponsorship for Specialist Registrar (SpR) post in Clinical Microbiology through the Equivalence route (2005)

Dr. Noyal Mariya Joseph

  • The "Best poster paper award" for the paper titled "Simple screening tests for detection of carbapenemases in clinical isolates of Gram negative non-fermentative bacteria" during the 2nd National conference of the Infectious disease society of India (IDSI), held at Hyderabad (2008).

Any other achievements

  1. Prof. Selvaraj Stephen is Chairman of Human Ethics Committee, Sri Manakula Vinayagar Medical College, Pondicherry.
  2. Prof. Selvaraj Stephen is the member of Animal Ethics committee of Sri Manakula Vinayagar Medical College, Pondicherry.
  3. Prof. Selvaraj Stephen is the Chairman of Animal Ethics committee of MGMC&RI, Pondicherry
  4. Prof. Joshy M. Easow is the Hospital Infection Control Officer of MGMC&RI, Pondicherry.
  5. Dr. Srirangaraj S, Assistant Professor, is the Deputy Controller of Examination of Sri Balaji Vidyapeeth University, Pondicherry.

 


The faculty from the department has actively been part of the many national and international conferences / CME / Workshops.

  • International: 8
  • National: 83

 


The department also gives significant importance to various research activities. Many research projects have been completed in the past years and currently several other new projects are being carried out.

Total Number of Research Publications by Faculty in Indexed Medical Journals: 69

Research information Ongoing Research Projects

ICMR AD-HOC Research Project

"Seroprevalence of Q Fever, Spotted Fever and Scrub Typhus in Pondicherry" Revised project proposal has been sent to ICMR for further action Prof. Selvaraj Stephen (Principal Investigator)

ICMR Student Research Projects (submitted for approval)

  1. Prevalence of rapidly growing (Runyons Group IV) atypical mycobacterial in the environment of high risk areas in a tertiary care hospital in Puducherry.
  2. Anti-HBsAg level amongst vaccinated health care workers of a teaching hospital in Puducherry.
  3. Etiological agents and risk factors of injection abscess.
  4. Isolation & biofilm formation of uropathogens causing urinary tract infection in catheterised patients.

Institutional Research Projects (MGMC&RI)

  1. Prevalence of ESBL producing gram negative bacilli isolated At MGMC & RI
  2. Seroprevalence of HIV Infection among the patients attending MGMCH & RI.
  3. Prevalence of Candida species in patients with chronic respiratory tract infection.
  4. Bacteriological study of Methicillin Resistant Staphylococcus aureus (MRSA) strains isolated at MGMC & Rl, Pondicherry
  5. Aerobic isolates in Ventilator-associated pneumonia from a tertiary care hospital in South India.
  6. Clinical and investigative profile of Liver abscess – an observative study
  7. Aerobic and anaerobic infection in patients with necrotizing fasciitis
  8. A prospective study on Clostridium difjicile associated diarrhea at a teaching hospital in Puducherry.
  9. Clinical and bacteriological profile of systemic and deep seated surgical infections with special emphasis on anaerobic bacteria.
  10. Prevalence of genital Chlamydia trachomatis infections in women by antigen and antibody detection methods.
  11. Study of aerobic bacteria and prevalence of ESBL producers in post-operative wound infection.