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The Department of Biochemistry, MGMCRI began its academic journey on a humble note in the year 2001 and since then has emerged as one of the largest and premier post graduate departments of the institute.

The department caters to the needs of the students and patients by imparting theoretical, practical skills to the students and providing quality, efficient and reliable laboratory service to the patients.

Biochemistry Biochemistry
PORTALS OF BIOCHEMISTRY DEPARTMENT THE GLOW WOULD CONTINUE FOREVER

 


The department is equipped with a more than adequate infrastructure. The academic / research / service activities of the department centre around the main block, Department of Biochemistry situated at the College premises and also the Clinical Biochemistry laboratory, housed in the Central laboratory block, at the hospital premises.

The students’ laboratory (main) is endowed with facilities that cater to the needs of the UG students in Medicine, Dentistry and Nursing. Practical sessions are held essentially during the afternoons of all week days. These sessions are essentially preceded by demonstration, wherein the taught are sensitized to the experiments being performed. The laboratory is backed by able and efficient technical and auxiliary personnel.
Research Laboratory.

The Research Laboratory housed adjacent to the students’ lab serves the needs of the PG and research workers.

In addition, a few tests in Clinical Biochemistry are being performed here. The M.D. and M.Sc. Biochemistry students get opportunities to plan and conduct experiments, besides carrying out their dissertation (thesis) work.

The Department of Biochemistry is actively involved in imparting theoretical and practical knowledge to the postgraduate students (M.Sc. Medical Biochemistry and M.Sc. Medical Microbiology – Faculty of Medicine).

CLINICAL BIOCHEMISTRY LABORATORY

The Clinical Biochemistry located in the hospital block boasts of state-of-the-art equipment and instrumentation. The equipments / instruments include fully automated chemistry analyzer, electro chemiluminiscence hormone analyzer, electrolyte analyzer based on ion selective electrodes (ISE), arterial blood gas analyzer, etc. Besides carrying out routine investigations (tests), the laboratory is also actively involved in the quantization of several diagnostically relevant analytes including drugs, hormones, etc. All analytical measurements are subject to strict quality check. The internal quality assurance program is facilitated by M/S Biorad USA. The external quality assessment (EQAS) is under the aegis of Clinical Biochemistry Laboratory, CMC, Vellore. The Clinical Biochemistry Laboratory, MGMCRI is highly rated by Clinical Biochemistry Lab, CMC, Vellore - a national Reference Lab.

*RELIABLE *FAST *AFFORDABLE

We are committed to delivering Reliable Fast and Affordable diagnostic services with uncompromising commitment to Quality, Ethics AND best in class laboratory practices.

SAMPLE JOURNEY

  1. Collection of the blood sample
  2. Labeling the blood sample
  3. Documenting the specimen
  4. Running the test
  5. Getting the results
  6. Collecting the reports

A. LESLEY PREMANAND, is presently working as the CENTRAL LAB INCHARGE, Mahatma Gandhi medical college and research institute, Puducherry Graduated from madras university. After completion of his post graduate diploma in laboratory technology from Loyola College, Chennai had served as lab in charge in Sapthagiri diagnostics Chennai, Osler diagnostics pvt.ltd Chennai, renowned diagnostics laboratories in Chennai for a period of 8years. After this he had joined MGMCRI as central lab in charge. From then he has taken up the maintenance ,Quality control, Quality check day today, rectifying the troubleshooting of all hi-tech equipment including Hitachi 902 and e 411 immunoassay analyzer etc,.

1. ROCHE /HITACHI 902

1.	ROCHE /HITACHI 902

The Roche / Hitachi 902 analyser is used for reporting test results pertaining to various body fluid samples to various body fluids samples for a wide range of analytes .

Following are the salient feature of the instruments

  • Fully automated
  • Discrete
  • Fully computerized
  • Analytes in serum /plasma, urine ,CSF, etc could be estimates
  • Analysis based on potentiometric and photometric assays.

Characteristics of the analyser

  • For 24 hrs use.
  • Sample bar code facility available.
  • Throughput of 200 tests/hr.
  • Throughput of 300 ISE tests /hr.
  • Refrigerated storage for 40 reagent cartarners.
  • Positions for routine ,stat ,calibrators ,Qc samples and wash solutions.
  • Provision for end point kinetic and isoenzyme analyser.Control system is used to control all instruments functions. Components include the LCD touch screen monitor and printer.

ROCHE / HITACHI 902 FULLY AUTOMATED ANALYZER

OPERATING PROCEDURE :
DAILY MAINTENANCE

  1. Wash cells & measurement and confirmation of cell blank.
  2. Clean instruments surface.
  3. Clean and adjust sample probe , reagent probe, and stirrer.
  4. Check hitergent
  5. Check the printer paper supply
  6. An incubation bath exchange is required daily.
  7. Reagent volume Check.
  8. Photometer Check.
  9. Check incubation bath, & reagent disk compartment Temperature.
  10. Check Conductivity METER.

PROGRAMMING : ( EASY MODE PROCEDURE )

  1. To touch easy mode on the home screen to display the easy 1 screen
  2. Then load the sample on to the sample disk in the position.
  3. Touch ID key to enter a sample ID number on the easy 2 screen.
  4. Enter the sample ID number in the sample ID field then touch to return to the easy 2 screen.
  5. To touch the individual test keys to select the test to be run on this sample.
  6. Then select all tests to be OK to press accept key.
  7. To touch start key to run the sample.
  8. To load the calibration and controls in the required position on the same disk.
  9. Routine results and control, calibration results are printed in the printer paper.
  10. Finally perform the wash procedure after daily routine analysis, then clean and adjust sample, reagent probe and stirrer.
  11. Finally power off the analyzer.

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2. COBAS e 411 IMMUNOASSAY ANALYSER

COBAS
The Roche diagnostic cobas e 411 analyzer represents the state-of-the-art fully automated, random access, software controlled system for immunoassay analyzer.

The analyzer has been designed for both quantitative and qualitative in vitro determination of analyses in body fluids using a wide variety of tests.



Following are the salient features of the instruments.

  • Bench top.
  • Minimum scope for manual errors.
  • Automatic entry of information related to assay reagents, calibrates and control
  • Barcode/ e-barcode facility.
  • Comprehensive assay processing, control unit.
  • Foolproof data transmission from and to the analyzer.
  • Evaluation of results, documentation and quality control are performed automatically by the software.

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3. Ion selective electrode based electrolyte analyser-Easylyte

Ion selective electrode The Easylyte analyser is based on ion selective electrode (ISE). The analyzer provides on accurate analysis of electrolytes in biological fluids including blood and urine.

The instrument measures sodium, potassium and chloride. In addition calcium/ph in biological fluids and also be measured using ISE technology. The flow through sodium and ph electrode contains glass tubing, specially formulated to be sensitive to sodium ions. The flow – through potassium and calcium electrodes emplay a plastic tube, incorporating neutral carrier ionophares

The flow –through chloride electrode includes a specially formulated plastic tube. The potential of each electrode is measured relative to fixed, stable voltage provided by the Ag/Agcl reference electrode. The relationship between the voltage developed and ion concentration as logarithm.

Salient Features

  • Automated batch sampling of serum, plasma or diluted urine.
  • Allows stat analysis of even blood samples.
  • Possesses a 24 position sample, tray that includes 21 sample position, one stat position and two quality control position.
  • Results are printed for each sample indicating tray and position numbers, as well as date and time of all analysis.
  • The operator can select a repeat analysis of each sample.

4. ABL 5 BLOOD GAS SYSTEM

ABL 5 BLOOD GAS SYSTEM
This system is otherwise known as blood Gases and blood PH test system. The ABL 5 instrument has been uniquely designed and intended for routine use on human blood. The instrument works with a small volume of blood and measures PH, Pco2 and Po2. In addition other parameters could be calculated. This state-of-the –art instrument is a product of the prestigious manufacturer, M/s Radiometer, copentrages.


Following are the salient features of ABL 5

  • Only a small volume of blood (85µl) is required for the accurate qualitative analysis
  • ABl 5 provides results for three calibrated parameters viz.PH, Po2, Pco2. In addition, eight derived parameters are available.
  • A comprehensive patient report enumerates patient data and measurements
  • Blood PH can be measured alternately; this is facilitated by the PH only mode and requires only 35µl of blood.
  • Quality control (QC ) has been enabled using the control solutions in qc mode. quality control reports list input data and measurement results.
  • Automatic calibration facility provides calibration based on two solutions and two premixed gases.
  • Every analysis (patient’s sample/calibration) is followed by “Rinse” which is executed automatically.

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5. SAPPHIRE 120 SEMI AUTOANALYSER

SAPPHIRE  120  SEMI AUTOANALYSERThe instrument is a semi auto analyzers with nine test methods viz, end point, multistandards, double wavelength, two point, kinetics, nephelometery, enzymatic standard, absorbance and sample blank.

The instrument possesses a resolution rate of 0.0001 absorbance (display) facilities for measuring analytes of different temperatures (room temperature 25°, 30°c and 37°c) are available.


Following are the salient features of sapphire 120 auto analyzer

  • The instrument is user friendly
  • The instrument possesses the latest micro processor and multifunction software.
  • An interactive touch LCD operation mode is available.
  • The presence of a sensitive photometer enables accurate measurement of analytes based on absorbance (A) or optical density (O.D)
  • The analyser can store the data of 1000 patients.

6. THE HUMAN CLOT JUNIOR

THE HUMAN CLOT JUNIOR

The humaclot junior is a single –channel optical coagulometer that is endused with the capacity to determine the basic/key parameters pertaining to the second stage of hemostat as (coagulation casecade) Citrated human plasma is required for this purpose.
Following are the salient features of the instruments

  • Prothrombin time (PT) activated partial prothrombin time (APTT) and thrombin time (TT) couled be analysed. Fibrinogen and D-Dimer concentration could also be facilitated on the instrument.
  • The incubation area has 8position for sample and 2 for reagent.
  • In general humaclot junior has been specifically designed for in –vitro coagulation assays featuring fiberin formation as the endpoint. In addition, immunoturbidometric test for D-Dimer measurement is also enabled.

REPORTING SYSTEM

As the TATA HMS software is installed all over the hospital, the reports from the central laboratory (bio-chemistry, pathology, microbiology) are issued in the printed format. The reports are immediately entered in the systems and a computer print out of the same is issued to the patient. Further these reports can be viewed in any of the OPD’s, theaters, casualty and in wards.

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Under Construction

 


Dr. A. R. Srinivasan, Associate Professor of Biochemistry

  1. Chaired “Nexus Biochemie” – an International Symposium in Biochemistry, held under the aegis of Department of Biochemistry, Christian Medical College (CMC), Vellore, in commemoration of 50 years of teaching Biochemistry at CMC, Vellore – October 2007.
  2. Panelist at the discussion titled ‘Do we need to restructure Medical Education to develop an integrated system unique to Indian needs’, held at MGMCRI, to commemorate the platinum jubilee year of the Medical Council of India (MCI) – July 2008.
     

 


Under Construction

 

 


THE DEPARTMENTAL OBJECTIVES

  1. To prepare need based and state-of-the-art curricula so as to help enhance the objectivity of the academic performance.
  2. To develop and promulgate teaching programs in basic, clinical and practical Biochemistry, keeping in view the rapidly changing global scenario, with reference to basic medical sciences, in general and Biochemistry in particular.
  3. To encourage problem based learning (PBL) among UG students, especially in the light of case oriented approach - in the realms of Clinical Biochemistry.
  4. To provide quality efficient and reliable laboratory results in the augmentation clinical services.
  5. To strictly adhere to internal and external quality control , with reference to the patient oriented service activities: sample collection & processing results and documentation.
  6. To keep updating the available existing knowledge in the realms of instrumentation, methodology and mode of functioning of the clerical biochemistry laboratory.

GOAL
The Postgraduate education in Biochemistry should enable the student to acquire an in-depth knowledge in Biochemistry and apply it for understanding health and disease. At the end of his/her training the student shall be able to take up a career in teaching institution or in Diagnostic work or in Research.

OBJECTIVES:
At the end of the 3 years training in Biochemistry the student is expected to:

Knowledge:

  1. Understand the concept of Biochemistry regarding biomolecules
  2. Elaborate the fundamental aspects of enzymology and its application in clinical medicine
  3. Integrate the various aspects of metabolism and their regulatory pathways. Explain the biochemical basis of inborn errors of metabolism with their associated sequelae
  4. Understand the role of nutrition in health and disease
  5. Explain the mechanisms involved in maintenance of body fluids and pH homeostasis
  6. Outline the molecular mechanisms of gene expression and regulation and application of molecular biological techniques and molecular diagnostics in medicine
  7. Discuss the molecular concept of body defense and its application in medicine
  8. Describe the biochemical basis of environmental health hazards and cancer.
  9. Have knowledge regarding the analysis of biological fluids for its chemical constituents and correlating the same in health and disease
  10. Describe the principles of various conventional and specialized laboratory investigations and instrumentation analysis and interpretation of given data in clinical diagnosis.
  11. Be familiar with literature survey, research methodology, biostatistics and computer skills

Skills:

  1. Plan and conduct lectures, practical demonstration, tutorial classes, and small group discussion on various biochemical topics for undergraduate students of medical and allied disciplines
  2. Develop skill as a self directed learner, recognize continuing educational needs, select and use appropriate learning resources
  3. Make use of conventional techniques / instruments to perform biochemical analysis relevant to clinical screening and diagnosis.
  4. Organize a laboratory set up to deliver optimum investigation support for patient care services.
  5. Perform biochemical  laboratory investigations and experiments relevant to research
  6. Critically review and comment on research papers and make oral presentations
  7. Prepare research protocols, conduct experimental studies, analyze and solve clinical problems